24.09.2009
Atalanta Bergamo [0 - 0] Catania Calcio
AS Bari [0 - 1] Cagliari Calcio 0-1 Nenê
FC Bologna [2 - 0] AS Livorno 1-0 Portanova 2-0 Di Vaio
AC Florenz [2 - 0] Sampdoria 1-0 Jovetic 2-0 Gilardino
Lazio Rom [1 - 2] FC Parma 0-1 Bojinov 1-1 Zarate 1-2 Amoruso (Pen)
US Palermo [3 - 3] AS Rom 0-1 Brighi 1-1 Budan 1-2 Burdisso 2-2 Miccoli 3-2 Nocerino 3-3 Totti (Pen)
AC Siena [0 - 0] Chievo Verona
Udinese Calcio [1 - 0] AC Mailand 1-0 Di Natale
Inter Mailand [3 - 1] SSC Neapel 1-0 Eto'o 2-0 Milito 3-0 Lucio 3-1 Lavezzi